Analytical Data
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Gene name
AGPAT5
- Application
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Alternative Names
AGPAT5; 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-acylglycerol-3-phosphate
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal.
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NUQ2
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Expression Region
1-364aa
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AA Sequence
MLLSLVLHTYSMRYLLPSVVLLGTAPTYVLAWGVWRLLSAFLPARFYQALDDRLYCVYQSMVLFFFENYTGVQILLYGDLPKNKENIIYLANHQSTVDWIVADILAIRQNALGHVRYVLKEGLKWLPLYGCYFAQHGGIYVKRSAKFNEKEMRNKLQSYVDAGTPMYLVIFPEGTRYNPEQTKVLSASQAFAAQRGLAVLKHVLTPRIKATHVAFDCMKNYLDAIYDVTVVYEGKDDGGQRRESPTMTEFLCKECPKIHIHIDRIDKKDVPEEQEHMRRWLHERFEIKDKMLIEFYESPDPERRKRFPGKSVNSKLSIKKTLPSMLILSGLTAGMLMTDAGRKLYVNTWIYGTLLGCLWVTIKA
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Molecular Weight
68.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGPAT5, or acylglycerol-3-phosphate acyltransferase 5, is a key enzyme involved in lipid metabolism, particularly in the synthesis of triglycerides and phospholipids. It plays a crucial role in regulating energy homeostasis and lipid storage within adipocytes. Understanding the function and regulation of AGPAT5 is essential due to its potential implications in various metabolic disorders, including obesity, type 2 diabetes, and non-alcoholic fatty liver disease. Recent studies have highlighted its involvement in lipid droplet formation and adipocyte differentiation, revealing AGPAT5 as a significant player in metabolic health. Furthermore, the development of recombinant AGPAT5 proteins has provided valuable tools for studying its enzymatic activity and regulatory mechanisms in vitro and in vivo. Investigating the structural and functional properties of AGPAT5, including its substrate specificity and interaction with other metabolic pathways, can offer insights into therapeutic strategies targeting lipid metabolism-related diseases. Overall, AGPAT5 holds promise for advancing our understanding of lipid biology and its associated disorders, making it a focus of ongoing research in the field of metabolic health.
