Analytical Data
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基因名
ST6GAL1
- Application
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别名
ST6GAL1;SIAT1;Beta-galactoside alpha-2.6-sialyltransferase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15907
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表达区间
27-406aa
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氨基酸序列
KEKKKGSYYDSFKLQTKEFQVLKSLGKLAMGSDSQSVSSSSTQDPHRGRQ TLGSLRGLAKAKPEASFQVWNKDSSSKNLIPRLQKIWKNYLSMNKYKVSY KGPGPGIKFSAEALRCHLRDHVNVSMVEVTDFPFNTSEWEGYLPKESIRT KAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNGAPTANFQQDVGTK TTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQNPDYN FFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGML GIIIMMTLCDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEK NLVKHLNQGTDEDIYLLGKATLPGFRTIHCVDHHHHHH
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ST6GAL1 gene encodes the enzyme ST6 beta-galactosamine alpha-2,6-sialyltransferase 1, which plays a crucial role in the sialylation of glycoproteins and glycosphingolipids. This enzymatic process is vital for various biological functions, including cell signaling, immune response, and cell-cell interactions. Abnormal ST6GAL1 activity has been implicated in several diseases, including cancer, where altered sialylation patterns can affect tumor progression and metastasis by influencing cancer cell behaviors and evasion of immune surveillance. Studies have shown that overexpression of ST6GAL1 can enhance the sialylation of glycoproteins, which may contribute to tumor aggressiveness. Consequently, understanding the structure and function of ST6GAL1 through recombinant protein studies is essential for elucidating its biological roles and potential therapeutic applications. Recombinant ST6GAL1 proteins can be utilized to investigate enzyme kinetics, substrate specificity, and the effects of various biochemical factors on sialylation processes. Additionally, these studies may lead to the development of novel biomarkers and therapeutic targets for diseases associated with aberrant sialylation. Overall, the research on ST6GAL1 recombinant proteins holds promise for advancing our knowledge of glycosylation mechanisms in health and disease, paving the way for innovative approaches in diagnostics and treatments.
