Analytical Data
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Gene name
CYP4F2
- Application
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Alternative Names
CYP4F2；Cytochrome P450 4F2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78329
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Expression Region
5-520aa
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AA Sequence
SLSWLGLWPVAASPWLLLLLVGASWLLAHVLAWTYAFYDNCRRLRCFPQPPRRNWFWGHQ GMVNPTEEGMRVLTQLVATYPQGFKVWMGPISPLLSLCHPDIIRSVINASAAIAPKDKFF YSFLEPWLGDGLLLSAGDKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASE GSACLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVSKRHHEILLHID FLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLL SKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKD REPKEIEWDDLAHLPFLTMCMKESLRLHPPVPVISRHVTQDIVLPDGRVIPKGIICLISV FGTHHNPAVWPDPEVYDPFRFDPENIKERSPLAFIPFSAGPRNCIGQTFAMAEMKVVLAL TLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS
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Molecular Weight
59.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP4F2, a member of the cytochrome P450 family, is a key enzyme involved in the metabolism of various endogenous and exogenous substances, including fatty acids, steroids, and drugs. Its role in the metabolism of vitamin K and anticoagulant drugs, such as warfarin, underscores its clinical significance, as genetic variations in the CYP4F2 gene can influence individual responses to these medications. Studies have demonstrated that polymorphisms in the CYP4F2 gene can lead to altered enzyme activity and subsequently affect drug efficacy and safety. Consequently, research into the recombinant CYP4F2 protein has gained momentum, with efforts focused on understanding its structure, function, and interaction with substrates and inhibitors. Characterizing the recombinant CYP4F2 protein is crucial for elucidating its metabolic pathways and for developing personalized medicine approaches to optimize drug dosing and minimize adverse effects. Furthermore, insights gained from this research can contribute to better therapeutic strategies for managing conditions that require anticoagulation therapy, ultimately enhancing patient outcomes.
