Analytical Data
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基因名
AFAP
- Application
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别名
AFAP 1; AFAP 110; AFAP; AFAP-110; Afap1; AFAP1_HUMAN; AFAP110; FLJ56849
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N556
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表达区间
1-730aa
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氨基酸序列
MEELIVELRL FLELLDHEYL TSTVREKKAV ITNILLRIQS SKGFDVKDHA QKQETANSLP APPQMPLPEI PQPWLPPDSG PPPLPTSSLP EGYYEEAVPL SPGKAPEYIT SNYDSDAMSS SYESYDEEEE DGKGKKTRHQ WPSEEASMDL VKDAKICAFL LRKKRFGQWT KLLCVIKDTK LLCYKSSKDQ QPQMELPLQG CNITYIPKDS KKKKHELKIT QQGTDPLVLA VQSKEQAEQW LKVIKEAYSG CSGPVDSECP PPPSSPVHKA ELEKKLSSER PSSDGEGVVE NGITTCNGKE QVKRKKSSKS EAKGTVSKVT GKKITKIISL GKKKPSTDEQ TSSAEEDVPT CGYLNVLSNS RWRERWCRVK DNKLIFHKDR TDLKTHIVSI PLRGCEVIPG LDSKHPLTFR LLRNGQEVAV LEASSSEDMG RWIGILLAET GSSTDPEALH YDYIDVEMSA SVIQTAKQTF CFMNRRVISA NPYLGGTSNG YAHPSGTALH YDDVPCINGS LKGKKPPVAS NGVTGKGKTL SSQPKKADPA AVVKRTGSNA AQYKYGKNRV EADAKRLQTK EEELLKRKEA LRNRLAQLRK ERKDLRAAIE VNAGRKPQAI LEEKLKQLEE ECRQKEAERV SLELELTEVK ESLKKALAGG VTLGLAIEPK SGTSSPQSPV FRHRTLENSP ISSCDTSDTE GPVPVNSAAV LKKSQAAPGS SPCRGHVLRK AKEWELKNGT
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分子量
106.04 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AFAP (Actin Filament Associated Protein) is a pivotal protein involved in various cellular processes linked to the cytoskeleton and cell signaling. Its research background is rooted in the understanding of how actin dynamics affect cell shape, motility, and intracellular transport. AFAP, characterized by its ability to associate with actin filaments, plays a crucial role in the organization and stabilization of the cytoskeleton, making it essential for cellular architecture and function. Studies have indicated that AFAP can also influence cell adhesion and migration, which is particularly relevant in cancer biology, as altered cell motility can lead to metastasis. Additionally, AFAP has been implicated in various cellular responses to mechanical stress, highlighting its importance in maintaining cellular integrity under different physiological conditions. Recent advancements in recombinant protein technology have enabled researchers to produce AFAP in significant quantities, facilitating in-depth investigations into its structural properties, interactions with other cytoskeletal proteins, and functional roles in broader biological systems. Understanding AFAP's mechanisms can shed light on its contributions to health and disease, offering potential therapeutic targets for conditions associated with cytoskeletal dysfunction. The ongoing research into AFAP not only enriches our knowledge of the actin cytoskeleton but also provides valuable insights into the molecular pathways that govern cellular behavior in both normal physiology and pathological states.
