Analytical Data
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Gene name
SPOP
- Application
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Alternative Names
SPOP;Speckle-type POZ Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43791
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Expression Region
1-374aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMSRVPSPPPPAEMSSGPVAESWCYTQIKVV KFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEES KDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKD WGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVK VPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFE HEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKY ALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHAS DVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPOP (Speckle-type POZ protein) is an E3 ubiquitin ligase that plays a critical role in the regulation of protein degradation and various cellular processes, including cell cycle progression, apoptosis, and transcriptional regulation. Its function is particularly significant in the context of cancer, where mutations or dysfunction in SPOP have been linked to the development and progression of several malignancies, especially prostate cancer. The identification of SPOP as a tumor suppressor suggested its potential as a therapeutic target, highlighting the importance of understanding its structural and functional characteristics. Research on recombinant SPOP proteins has gained momentum as scientists seek to elucidate its mechanisms of action, regulatory pathways, and interactions with substrates like the androgen receptor. Moreover, recombinant SPOP proteins provide a valuable tool for studying the effects of mutations and post-translational modifications on its activity. Characterizing SPOP through protein engineering and functional assays can help discover new insights into its involvement in cancer biology and pave the way for novel therapeutic strategies. This growing body of research underscores the need for detailed analysis of SPOP's roles at the molecular level, aiming to translate findings into clinical applications for cancer treatment and prevention.
