Analytical Data
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Gene name
ADCK4
- Application
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Alternative Names
COQ8B; ADCK4; Atypical kinase COQ8B. mitochondrial; AarF domain-containing Protein kinase 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96D53
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Expression Region
1-544aa
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AA Sequence
MWLKVGGLLRGTGGQLGQTVGWPCGALGPGPHRWGPCGGSWAQKFYQDGPGRGLGEEDIRRAREARPRKTPRPQLSDRSRERKVPASRISRLANFGGLAVGLGLGVLAEMAKKSMPGGRLQSEGGSGLDSSPFLSEANAERIVQTLCTVRGAALKVGQMLSIQDNSFISPQLQHIFERVRQSADFMPRWQMLRVLEEELGRDWQAKVASLEEVPFAAASIGQVHQGLLRDGTEVAVKIQYPGIAQSIQSDVQNLLAVLKMSAALPAGLFAEQSLQALQQELAWECDYRREAACAQNFRQLLANDPFFRVPAVVKELCTTRVLGMELAGGVPLDQCQGLSQDLRNQICFQLLTLCLRELFEFRFMQTDPNWANFLYDASSHQVTLLDFGASREFGTEFTDHYIEVVKAAADGDRDCVLQKSRDLKFLTGFETKAFSDAHVEAVMILGEPFATQGPYDFGSGETARRIQDLIPVLLRHRLCPPPEETYALHRKLAGAFLACAHLRAHIACRDLFQDTYHRYWASRQPDAATAGSLPTKGDSWVDPS
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ADCK4 (akin to cyclic nucleotide phosphodiesterases and adenylyl cyclases) is a member of a family of proteins involved in mitochondrial function and metabolism, particularly in the context of coenzyme Q10 (CoQ10) biosynthesis. Mutations in the ADCK4 gene have been linked to a range of disorders, notably the autosomal recessive cerebellar ataxia associated with CoQ10 deficiency, which highlights its crucial role in cellular energy production and oxidative stress management. Research has shown that the ADCK4 protein is essential for the proper functioning of mitochondrial respiratory chain complexes, making it vital for ATP synthesis and cellular health. Given its role in CoQ10 synthesis, further investigations into the biochemical pathways involving ADCK4 could provide critical insights into mitochondrial dysfunction-related diseases. Additionally, the development of ADCK4 recombinant protein is significant for studying its functional properties, elucidating its interactions with other metabolic pathways, and exploring potential therapeutic interventions for conditions associated with ADCK4 deficiencies. Understanding the structure and function of ADCK4 at a molecular level may pave the way for innovative strategies to treat or manage diseases linked to mitochondrial dysfunction, thereby contributing to advancements in precision medicine and targeted therapies.
