Analytical Data
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Gene name
SPG21
- Application
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Alternative Names
SPG21;ACP33;Maspardin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NZD8
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Expression Region
1-308aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS LGISQEEQ
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPG21, also known as spatacsin, is a gene associated with hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by progressive weakness and stiffness of the legs. Mutations in the SPG21 gene are linked to a specific form of autosomal recessive HSP, which has been observed in various populations. Understanding the function of SPG21 and its encoded protein is crucial for elucidating the molecular mechanisms underlying HSP and developing potential therapeutic strategies. Recent advances in recombinant DNA technology have enabled the production of SPG21 as a recombinant protein, which facilitates the detailed study of its structure and function. Characterizing SPG21's protein interactions, cellular localization, and biological roles can enhance our understanding of its involvement in neurodegeneration. Additionally, exploring the consequences of pathogenic mutations on the protein's stability and function may provide insights into disease pathogenesis and identify potential biomarkers for early diagnosis. Ultimately, further research on SPG21 could pave the way for innovative treatments aimed at mitigating the effects of this debilitating condition, improving the quality of life for affected individuals.
