Analytical Data
-
Gene name
HDAC10
- Application
-
Alternative Names
HDAC10；Polyamine deacetylase HDAC10
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q969S8
-
Expression Region
1-482aa
-
AA Sequence
MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRL SAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTF HCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNV AIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHG RFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLA FEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAV LEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQ APHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLD QPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHES LAREEALTALGKLLYLLDGMLDGQVNSGIAAT
-
Molecular Weight
71 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Histone deacetylase 10 (HDAC10) is a member of the HDAC family, which plays a crucial role in the regulation of gene expression by removing acetyl groups from histones, leading to chromatin condensation and transcriptional repression. Unlike other HDACs, HDAC10 is primarily localized in the cytoplasm and has been implicated in various cellular processes, including cell cycle regulation, differentiation, and apoptosis. Recent studies have highlighted its involvement in several diseases, particularly cancer, where altered HDAC activity contributes to tumorigenesis and poor prognosis. Given its distinct functional characteristics and the potential for HDAC inhibitors as therapeutic agents, HDAC10 has garnered attention as a novel target for drug development. The need for a better understanding of HDAC10's structure and function has propelled research into its recombinant protein expression and purification, allowing for detailed biochemical and biophysical analyses. By utilizing recombinant HDAC10, researchers aim to elucidate its enzymatic properties, identify its substrates, and explore its regulatory mechanisms, thereby providing insights that could lead to the development of targeted therapies for diseases associated with HDAC dysregulation. This research is pivotal in advancing our knowledge of epigenetic regulation and its implications for health and disease.
