Analytical Data
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Gene name
OFD1
- Application
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Alternative Names
71 7A; 717A; CXorf5; JBTS10; Ofd1; OFD1_HUMAN; oral facial digital syndrome 1; Oral-facial-digital syndrome 1 protein; OTTHUMP00000022941; Protein 71-7A; RGD1562231; SGBS2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75665
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Expression Region
1-550 aa
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AA Sequence
MLNEKVKEMSDYSLLKEEKLELLAQNKLLKQQLEESRNENLRLLNRLAQPAPELAVFQKELRKAEKAIVVEHEEFESCRQALHKQLQDEIEHSAQLKAQILGYKASVKSLTTQVADLKLQLKQTQTALENEVYCNPKQSVIDRSVNGLINGNVVPCNGEISGDFLNNPFKQENVLARMVASRITNYPTAWVEGSSPDSDLEFVANTKARVKELQQEAERLEKAFRSYHRRVIKNSAKSPLAAKSPPSLHLLEAFKNITSSSPERHIFGEDRVVSEQPQVGTLEERNDVVEALTGSAASRLRGGTSSRRLSSTPLPKAKRSLESEMYLEGLGRSHIASPSPCPDRMPLPSPTESRHSLSIPPVSSPPEQKVGLYRRQTELQDKSEFSDVDKLAFKDNEEFESSFECVDQKQIEEQKEEEKIREQQVKERRQREERRQSNLQEVLERERRELEKLYQERKMIEESLKIKIKKELEMENELEMSNQEIKDKSAHSENPLEKYMKIIQQEQDQESADKSSKKMVQEGSLVDTLQSSDKVESLTGFSHEELDDSW
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Molecular Weight
89.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
OFD1 (Orofaciodigital syndrome 1) is a ciliary protein encoded by the OFD1 gene, primarily involved in the development and function of cilia, which are essential for various cellular signaling pathways. Mutations in the OFD1 gene are associated with Orofaciodigital syndrome type 1, a rare genetic disorder characterized by oral, facial, and digital abnormalities, often accompanied by renal and neurological complications. Research into OFD1 and its associated protein has gained momentum due to its critical role in ciliary biology, which is increasingly recognized for its significance in various diseases, including ciliopathies and cancer. Understanding the structure and function of recombinant OFD1 protein is pivotal for elucidating its mechanisms in ciliary assembly and the pathogenic processes behind OFD1-related disorders. Recent studies have focused on producing and characterizing recombinant OFD1 protein to explore its interactions with other ciliary components and to determine how its dysfunction contributes to the phenotypic manifestations of disease. Advances in protein engineering and expression systems have facilitated the generation of these recombinant proteins, enabling researchers to investigate their biochemical properties, cellular localization, and functional roles in cilia. This knowledge may lead to novel therapeutic strategies aimed at mitigating the effects of OFD1 mutations and improving the quality of life for affected individuals. Overall, the study of recombinant OFD1 protein represents a crucial step in unraveling the complex biology of cilia and their associated disorders.
