Analytical Data
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基因名
OGFOD1
- Application
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别名
2 oxoglutarate and iron dependent oxygenase domain containing 1; 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1; FLJ10826; KIAA1612; OGFD1_HUMAN; OGFOD1; Termination and polyadenylation 1 homolog; TPA1; TPA1 termination and polyadenylation 1 homolog
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8N543
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表达区间
1-542 aa
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氨基酸序列
MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS HEVIVMDMDP FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ SDDLKKRREP HISTLRKILF EDFRSWLSDI SKIDLESTID MSCAKYEFTD ALLCHDDELE GRRIAFILYL VPPWDRSMGG TLDLYSIDEH FQPKQIVKSL IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP SLTRPPNYFE PPIPRSPHIP QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK PEKFTKVCEA LEHGHVEWSS RGPPNKRFYE KAEESKLPEI LKECMKLFRS EALFLLLSNF TGLKLHFLAP SEEDEMNDKK EAETTDITEE GTSHSPPEPE NNQMAISNNS QQSNEQTDPE PEENETKKES SVPMCQGELR HWKTGHYTLI HDHSKAEFAL DLILYCGCEG WEPEYGGFTS YIAKGEDEEL LTVNPESNSL ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE
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分子量
63.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OGFOD1 (Oxygen-Dependent Fumarate Hydratase Domain 1) is a protein that has garnered attention due to its potential role in various biological processes, particularly in cellular response to hypoxia and regulation of protein stability. Studies have indicated that OGFOD1 acts as a novel dioxygenase, which modifies hypoxia-induced factors, thus influencing cellular pathways related to adaptation to low oxygen levels. The protein has also been implicated in several pathophysiological conditions, including cancer, where it may play a role in tumor progression and metastasis. The understanding of OGFOD1's function is further complicated by its involvement in the post-translational modification of proteins, particularly by affecting the hydroxylation of proline residues, which is crucial for the stability and function of numerous proteins. This has led to increased interest in OGFOD1 as a potential therapeutic target for diseases characterized by altered oxygen sensing or signaling pathways. As researchers explore the biochemical properties and interactions of OGFOD1, the characterization of its recombinant form has become essential for elucidating its functional mechanisms. This research not only provides insights into the fundamental roles of OGFOD1 in cellular physiology but also opens avenues for developing novel therapeutic strategies aimed at modulating its activity in disease contexts. Overall, the study of OGFOD1 and its recombinant protein is critical for advancing our understanding of cellular adaptability and the molecular basis of diseases associated with oxygen homeostasis.
