Analytical Data
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Gene name
SMARCA4
- Application
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Alternative Names
SMARCA4;BAF190A;BRG1;SNF2B;Transcription activator BRG1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51532
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Expression Region
700-1246aa
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AA Sequence
EVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAF
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Molecular Weight
68.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMARCA4, also known as BRG1, is a crucial component of the SWI/SNF chromatin remodeling complex, playing a significant role in the regulation of gene expression and maintaining genomic integrity. Alterations in SMARCA4 have been implicated in various cancers, particularly lung cancer and certain pediatric tumors, highlighting its function as a potential tumor suppressor. Research on SMARCA4 has gained momentum due to its involvement in critical cellular processes such as proliferation, differentiation, and DNA repair. Recurrent mutations or aberrant expression of SMARCA4 can disrupt the SWI/SNF complex's function, leading to altered chromatin accessibility and subsequent deregulation of oncogenes and tumor suppressor genes. Consequently, the study of SMARCA4 recombinant proteins is essential for understanding the molecular mechanisms underlying these alterations and their effects on tumorigenesis. By investigating the structure and function of SMARCA4 in a recombinant form, researchers aim to elucidate its role in chromatin dynamics and explore potential therapeutic targets. These studies may pave the way for developing novel strategies to combat cancers associated with SMARCA4 dysfunction by restoring normal chromatin remodeling activities.
