Analytical Data
-
基因名
Smad3
- Application
-
别名
SMAD3;MADH3;Mothers against decapentaplegic homolog 3
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P84022
-
表达区间
1-425aa
-
氨基酸序列
MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDE LEKAITTQNV NTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLH SHHELRAMELCEFAFNMKKDEV CVNPYHYQRVETPVLPPVLVPRHTEI PAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETP PPGYLSEDGETSDH QMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRV GETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGV RLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFN NQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPC WIELHLNGPLQWLDKVLTQMGSPSIRCSSVS
-
分子量
77 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Smad3 is a critical intracellular signaling protein that plays a pivotal role in the transforming growth factor-beta (TGF-β) superfamily signaling pathway, which is essential for various cellular processes such as proliferation, differentiation, and apoptosis. Research into Smad3 has gained prominence due to its implications in numerous pathological conditions, including fibrosis, cancer, and cardiovascular diseases. As an integral part of the TGF-β signaling cascade, Smad3 functions by transducing extracellular signals from TGF-β ligands to the nucleus, where it regulates gene expression. This process is vital for maintaining tissue homeostasis and responding to environmental cues. Recombinant Smad3 protein is therefore of substantial interest in both basic and applied research, as it allows scientists to dissect the intricate mechanisms of TGF-β signaling, explore its functional roles in various cellular contexts, and assess its potential as a therapeutic target. The generation of high-purity, active recombinant Smad3 enables detailed studies on its interactions with other signaling molecules, post-translational modifications, and its involvement in developmental processes. Understanding the role of Smad3 could pave the way for novel strategies in treating diseases influenced by aberrant TGF-β signaling, thus making it a focal point for ongoing research in molecular biology and medicine.
