Analytical Data
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Gene name
Smad3
- Application
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Alternative Names
SMAD3;MADH3;Mothers against decapentaplegic homolog 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P84022
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Expression Region
1-425aa
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AA Sequence
MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDE LEKAITTQNV NTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLH SHHELRAMELCEFAFNMKKDEV CVNPYHYQRVETPVLPPVLVPRHTEI PAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETP PPGYLSEDGETSDH QMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRV GETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGV RLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFN NQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPC WIELHLNGPLQWLDKVLTQMGSPSIRCSSVS
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Molecular Weight
77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Smad3 is a critical intracellular signaling protein that plays a pivotal role in the transforming growth factor-beta (TGF-β) superfamily signaling pathway, which is essential for various cellular processes such as proliferation, differentiation, and apoptosis. Research into Smad3 has gained prominence due to its implications in numerous pathological conditions, including fibrosis, cancer, and cardiovascular diseases. As an integral part of the TGF-β signaling cascade, Smad3 functions by transducing extracellular signals from TGF-β ligands to the nucleus, where it regulates gene expression. This process is vital for maintaining tissue homeostasis and responding to environmental cues. Recombinant Smad3 protein is therefore of substantial interest in both basic and applied research, as it allows scientists to dissect the intricate mechanisms of TGF-β signaling, explore its functional roles in various cellular contexts, and assess its potential as a therapeutic target. The generation of high-purity, active recombinant Smad3 enables detailed studies on its interactions with other signaling molecules, post-translational modifications, and its involvement in developmental processes. Understanding the role of Smad3 could pave the way for novel strategies in treating diseases influenced by aberrant TGF-β signaling, thus making it a focal point for ongoing research in molecular biology and medicine.
