Analytical Data
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基因名
Smad2
- Application
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别名
SMAD2;MADH2;MADR2;Mothers against decapentaplegic homolog 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15796
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表达区间
1-467aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPSTCSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLVPRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYYELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGCNLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS
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分子量
54 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Smad2 is a crucial intracellular signaling mediator in the transforming growth factor-beta (TGF-β) superfamily signaling pathway, which plays a vital role in regulating various cellular processes, including proliferation, differentiation, and apoptosis. Upon binding of TGF-β to its receptor, Smad2 is phosphorylated, leading to its activation and subsequent translocation to the nucleus, where it regulates gene expression by interacting with other transcription factors. The study of recombinant Smad2 protein has gained significant attention in recent years due to its importance in understanding the molecular mechanisms underlying developmental biology, tissue homeostasis, and tumorigenesis. By expressing and purifying recombinant Smad2, researchers can investigate its functional properties, binding interactions, and post-translational modifications. Moreover, examining Smad2 in the context of various pathological conditions, such as cancer and fibrosis, can reveal insights into its role as a potential therapeutic target. The ability to produce large quantities of recombinant Smad2 also opens opportunities for drug discovery and bioengineering applications, making it a valuable subject of research in the fields of molecular biology and biotechnology. Understanding the intricacies of Smad2 signaling pathways will not only enhance our knowledge of cellular functions but also contribute to the development of novel therapeutic strategies for diseases associated with dysregulated TGF-β signaling.
