Cat: PA2000-9826

Recombinant Human NXF5 Protein,His

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Analytical Data

  • Gene name

    NXF5

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    NXF5; TAPL1; Nuclear RNA export factor 5; TAP-like protein 1; TAPL-1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9H1B4

  • Expression Region

    1-397 aa

  • AA Sequence

    MRRNTQDENM RKWFKVTIPY GIKYDKAWLM NSIQSNCSVP FTPVDFHYIR NRACFFVQVA SAASALKDVS YKIYDDENQK ICIFVSHFTA PYSVKNKLKP GQMEMLKLTM NKRYNVSQQA LDLQNLRFDP DLMGRDIDII LNRRNCMAAT LKITERNFPE LLSLNLCNNK LYQLDGLSDI TEKAPKVKTL NLSKNKLESA WELGKVKGLK LEELWLEGNP LCSTFSDQSA YVSAIRDCFP KLLRLDGREL SAPVIVDIDS SETMKPCKEN FTGSETLKHL VLQFLQQSNL CKYFKDSRNI KILKDPYLQR KLLKHTKCPR NVDSLSALPE TQHDFTSILV DMWYQTVNTC FLPRAGPESQ RWWCLLSLKW KDGLRVLILP SCGPSSLPLA AIPVCAS

  • Molecular Weight

    45.6 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

NXF5, a member of the nucleocytoplasmic transport factor family, plays a crucial role in the regulation of mRNA export and diverse cellular processes. Research into NXF5 has gained momentum due to its implications in various diseases, including cancer and neurological disorders. Understanding the structure and function of NXF5 is essential, as it may offer insights into how cellular transport mechanisms influence gene expression and cellular homeostasis. Recent studies have focused on characterizing the recombinant NXF5 protein to elucidate its biological functions and interactions with other proteins involved in nucleocytoplasmic transport. By employing techniques such as protein expression in heterologous systems and purification methods, researchers aim to generate a functional NXF5 protein for in vitro studies. Insights gained from this research can enhance our understanding of the molecular pathways governed by NXF5 and may pave the way for potential therapeutic interventions targeting aberrant mRNA transport in diseases. Overall, the exploration of NXF5 as a recombinant protein represents a promising frontier in cell biology and disease research, highlighting the intricate relationship between nucleocytoplasmic transport and overall cellular health.

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