Analytical Data
-
基因名
SIRT3
- Application
-
别名
SIRT3;SIR2L3;NAD-dependent Protein deacetylase sirtuin-3. mitochondrial
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9NTG7
-
表达区间
118-399aa
-
氨基酸序列
MGSSHHHHHHSSGLVPRGSHMSDKGKLSLQDVAELIRARACQRVVVMVGA GISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFF HNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERV SGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGV VKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRS SVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDL VQRETGKLDGPDK
-
分子量
34 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SIRT3, a member of the sirtuin family of proteins, is a mitochondrial deacetylase that plays a crucial role in cellular metabolism and stress response. Research on SIRT3 has gained momentum due to its involvement in various physiological processes, including regulation of mitochondrial function, oxidative stress response, and control of energy metabolism. Malfunctions in SIRT3 have been linked to several age-related diseases, such as obesity, diabetes, and neurodegenerative disorders. The ability of SIRT3 to deacetylate key metabolic enzymes emphasizes its potential as a therapeutic target for metabolic syndromes. Additionally, SIRT3 has been implicated in the modulation of mitochondrial dynamics and apoptosis, further underscoring its significance in maintaining cellular homeostasis. The recombinant protein form of SIRT3 enables detailed mechanistic studies, allowing researchers to dissect its enzymatic functions and interactions with various substrates. Understanding the structural and functional characteristics of recombinant SIRT3 can provide insights into its regulatory mechanisms and facilitate the development of SIRT3-targeted therapies. Thus, ongoing research into SIRT3 is pivotal for uncovering its therapeutic potential in the prevention and treatment of metabolic and age-associated diseases.
