Analytical Data
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Gene name
NUP54
- Application
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Alternative Names
54 kDa nucleoporin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z3B4
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Expression Region
1-507 aa
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AA Sequence
MAFNFGAPSGTSGTAAATAAPAGGFGGFGTTSTTAGSAFSFSAPTNTGTTGLFGGTQNKGFGFGTGFGTTTGTSTGLGTGLGTGLGFGGFNTQQQQQTTLGGLFSQPTQAPTQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGYFNNNIPPVEFTQENPFCRFKAVGYSCMPSNKDEDGLVVLVFNKKETEIRSQQQQLVESLHKVLGGNQTLTVNVEGTKTLPDDQTEVVIYVVERSPNGTSRRVPATTLYAHFEQANIKTQLQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFKELLRRLKVQDQMTKQHQTRLDIISEDISELQKNQTTSVAKIAQYKRKLMDLSHRTLQVLIKQEIQRKSGYAIQADEEQLRVQLDTIQGELNAPTQFKGRLNELMSQIRMQNHFGAVRSEERYYIDADLLREIKQHLKQQQEGLSHLISIIKDDLEDIKLVEHGLNETIHIRGGVFS
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Molecular Weight
62.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NUP54, a key component of the nuclear pore complex (NPC), plays a crucial role in regulating nucleocytoplasmic transport, which is essential for various cellular processes, including gene expression, cell cycle progression, and signal transduction. The NPC is composed of multiple nucleoporins, with NUP54 being involved in forming the central channel through which macromolecules, such as RNA and proteins, are transported between the nucleus and cytoplasm. Research on NUP54 has garnered attention due to its potential implications in various diseases, including cancer and viral infections, where disruption of nuclear transport mechanisms can influence cell proliferation and pathogen replication. The production of recombinant NUP54 protein allows for in-depth studies of its structure and function, facilitating the understanding of the molecular mechanisms underlying its role in the NPC. Moreover, the characterization of NUP54 and its interactions with other nucleoporins and proteins can provide insights into the biogenesis and maintenance of nuclear pore complexes. Additionally, the study of NUP54's role in disease contexts, such as its expression levels in tumors or its interaction with viral proteins, may open new avenues for therapeutic strategies targeting nucleocytoplasmic transport processes. Overall, the research on NUP54 recombinant protein is vital for elucidating the complexities of nuclear transport and its consequences for cellular health and disease.
