Analytical Data
-
基因名
SPR
- Application
-
别名
SPR;Sepiapterin reductase
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P35270
-
表达区间
1-261aa
-
氨基酸序列
MEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK
-
分子量
75.8 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPR (Surface Plasmon Resonance) has emerged as a pivotal technology in the study of biomolecular interactions, particularly for recombinant proteins. Recombinant proteins, produced through genetic engineering techniques, are fundamental in various applications including therapeutics, diagnostics, and research. The ability to analyze the real-time interaction dynamics of these proteins with other biomolecules is crucial for understanding their functional mechanisms. SPR provides a label-free, sensitive method for monitoring these interactions by measuring changes in refractive index near a sensor surface, enabling the determination of binding kinetics and affinities. This technology facilitates the screening of protein-protein, protein-ligand, and protein-DNA interactions, paving the way for drug discovery and design. Recent advancements in SPR instrumentation, such as enhanced sensitivity and multiplexing capabilities, have further expanded its application spectrum. Understanding how recombinant proteins interact within biological systems not only informs the development of novel therapeutics but also aids in the design of protein-based biosensors and biomaterials. As such, the integration of SPR in recombinant protein research represents a significant advancement in the field of molecular biology and biochemistry, contributing to innovative solutions for contemporary health and scientific challenges.












