Analytical Data
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基因名
NPHS2
- Application
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别名
NPHS2
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NP85
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表达区间
1-315 aa
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氨基酸序列
MERRARSSSRESRGRGGRTPHKENKRAKAERSGGGRGRQEAGPEPSGSGRAGTPGEPRAPAATVVDVDEVRGSGEEGTEVVALLESERPEEGTKSSGLGACEWLLVLISLLFIIMTFPFSIWFCVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML
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分子量
60.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of NPHS2 (Nephrosis 2), a gene that encodes the protein podocin, is crucial in understanding its role in kidney function and nephrotic syndrome. Podocin is predominantly expressed in the podocytes, specialized cells in the kidneys that are essential for the filtration barrier's integrity. Mutations in NPHS2 are linked to familial nephrotic syndrome, leading to massive proteinuria, hypoproteinemia, and edema, which can severely affect patients' quality of life. Researchers aim to explore the structure-function relationship of NPHS2 by producing recombinant podocin protein, allowing for detailed investigations into its biochemical properties and interactions with other signaling molecules within the podocyte. Such studies are vital to elucidate the mechanisms underlying podocyte function and how disruptions caused by genetic mutations can lead to disease. The recombinant expression of NPHS2 offers a valuable tool for developing therapeutic strategies targeting nephrotic syndrome and enhancing our understanding of podocyte biology, potentially leading to novel interventions for patients suffering from kidney diseases.
