Analytical Data
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Gene name
NOL4
- Application
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Alternative Names
NOL4; NOLP; HRIHFB2255Nucleolar protein 4; Nucleolar-localized protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94818
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Expression Region
1-638 aa
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AA Sequence
MESERDMYRQ FQDWCLRTYG DSGKTKTVTR KKYERIVQLL NGSESSSTDN AKFKFWVKSK GFQLGQPDEV RGGGGGAKQV LYVPVKTTDG VGVDEKLSLR RVAVVEDFFD IIYSMHVETG PNGEQIRKHA GQKRTYKAIS ESYAFLPREA VTRFLMSCSE CQKRMHLNPD GTDHKDNGKP PTLVTSMIDY NMPITMAYMK HMKLQLLNSQ QDEDESSIES DEFDMSDSTR MSAVNSDLSS NLEERMQSPQ NLHGQQDDDS AAESFNGNET LGHSSIASGG THSREMGDSN SDGKTGLEQD EQPLNLSDSP LSAQLTSEYR IDDHNSNGKN KYKNLLISDL KMEREARENG SKSPAHSYSS YDSGKNESVD RGAEDLSLNR GDEDEDDHED HDDSEKVNET DGVEAERLKA FNMFVRLFVD ENLDRMVPIS KQPKEKIQAI IDSCRRQFPE YQERARKRIR TYLKSCRRMK RSGFEMSRPI PSHLTSAVAE SILASACESE SRNAAKRMRL ERQQDESAPA DKQCKPEATQ ATYSTSAVPG SQDVLYINGN GTYSYHSYRG LGGGLLNLND ASSSGPTDLS MKRQLATSSG SSSSSNSRPQ LSPTEINAVR QLVAGYRESA AFLLRSADEL ENLILQQN
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Molecular Weight
71.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NOL4, a nucleolar protein, has gained attention in recent years due to its potential role in cellular processes such as ribosome biogenesis, RNA metabolism, and stress response. This protein is part of the nucleolar structure, which is critical for the assembly of ribosomal RNA and proteins into ribosomes, essential for protein synthesis in all living cells. Abnormalities in NOL4 expression levels have been linked to various diseases, including cancer, highlighting its importance in cellular homeostasis and pathology. Studies have demonstrated that NOL4 interacts with several key proteins involved in cell cycle regulation and apoptosis, suggesting that it may serve as a crucial regulator in these processes. Furthermore, recent advancements in recombinant protein technology have facilitated the production and purification of NOL4, allowing researchers to investigate its structure-function relationships and mechanisms of action in detail. Understanding the precise roles of NOL4 in cellular functions could provide insights into its potential as a therapeutic target and biomarker in disease contexts. Consequently, ongoing research focuses on elucidating the molecular interactions of NOL4 and its implications in health and disease, paving the way for novel strategies in medical intervention.
