Analytical Data
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Gene name
NOL10
- Application
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Alternative Names
FLJ13938; FLJ14075; H_NH0074G24.1; NOL 10; Nol10; NOL10_HUMAN; Nucleolar protein 10; Polyglutamine binding protein 5; PQBP5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BSC4
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Expression Region
1-688 aa
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AA Sequence
MQVSSLNEVK IYSLSCGKSL PEWLSDRKKR ALQKKDVDVR RRIELIQDFE MPTVCTTIKV SKDGQYILAT GTYKPRVRCY DTYQLSLKFE RCLDSEVVTF EILSDDYSKI VFLHNDRYIE FHSQSGFYYK TRIPKFGRDF SYHYPSCDLY FVGASSEVYR LNLEQGRYLN PLQTDAAENN VCDINSVHGL FATGTIEGRV ECWDPRTRNR VGLLDCALNS VTADSEINSL PTISALKFNG ALTMAVGTTT GQVLLYDLRS DKPLLVKDHQ YGLPIKSVHF QDSLDLILSA DSRIVKMWNK NSGKIFTSLE PEHDLNDVCL YPNSGMLLTA NETPKMGIYY IPVLGPAPRW CSFLDNLTEE LEENPESTVY DDYKFVTKKD LENLGLTHLI GSPFLRAYMH GFFMDIRLYH KVKLMVNPFA YEEYRKDKIR QKIEETRAQR VQLKKLPKVN KELALKLIEE EEEKQKSTWK KKVKSLPNIL TDDRFKVMFE NPDFQVDEES EEFRLLNPLV SKISEKRKKK LRLLEQQELR EKEEEEEPEG KPSDAESSES SDDEKAWVEE VRKQRRLLQQ EEKVKRQERL KEDQQTVLKP QFYEIKAGEE FRSFKDSATK QKLMNKTLED RLKIEAKNGT LSVSDTTVGS KQLTFTLKRS EQQKKQQEAE KLHRQERKRL RRSAGHLKSR HKRGRSFH
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Molecular Weight
80.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NOL10, a member of the nucleolar protein family, has garnered attention due to its potential role in ribosome biogenesis and cellular stress responses. Recent studies indicate that NOL10 is implicated in the regulation of cell proliferation and apoptosis, suggesting its significance in various biological processes, including tumorigenesis. Its expression levels have been correlated with several cancer types, making it a candidate for understanding cancer mechanisms and potential therapeutic interventions. Moreover, NOL10 is believed to interact with other nucleolar proteins, playing a critical role in maintaining nucleolar integrity and function. The reconstitution of NOL10 as a recombinant protein enables detailed biochemical and biophysical characterizations, which are essential for deciphering its functional properties and interactions. This approach has opened avenues for studying the signaling pathways associated with NOL10, offering insights into its contribution to nucleolar dynamics and pathology. Understanding the structural and functional aspects of NOL10 may provide valuable information for developing novel strategies in cancer treatment and further elucidating the complexities of nucleolar functions in cellular homeostasis. As research in this area progresses, NOL10 could emerge as a significant molecular target for therapeutic development, particularly in oncological applications.
