Analytical Data
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Gene name
NMD3
- Application
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Alternative Names
60S ribosomal export protein NMD3; CGI 07; FLJ21053; hNMD3; NMD3; NMD3 homolog ; NMD3 homolog (S. cerevisiae); NMD3 ribosome export adaptor; NMD3. S. cerevisiae. homolog of; NMD3_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96D46
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Expression Region
1-503 aa
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AA Sequence
MEYMAESTDR SPGHILCCEC GVPISPNPAN ICVACLRSKV DISQGIPKQV SISFCKQCQR YFQPPGTWIQ CALESRELLA LCLKKIKAPL SKVRLVDAGF VWTEPHSKRL KVKLTIQKEV MNGAILQQVF VVDYVVQSQM CGDCHRVEAK DFWKAVIQVR QKTLHKKTFY YLEQLILKYG MHQNTLRIKE IHDGLDFYYS SKQHAQKMVE FLQCTVPCRY KASQRLISQD IHSNTYNYKS TFSVEIVPIC KDNVVCLSPK LAQSLGNMNQ ICVCIRVTSA IHLIDPNTLQ VADIDGSTFW SHPFNSLCHP KQLEEFIVME CSIVQDIKRA AGAGMISKKH TLGEVWVQKT SEMNTDKQYF CRTHLGHLLN PGDLVLGFDL ANCNLNDEHV NKMNSDRVPD VVLIKKSYDR TKRQRRRNWK LKELAREREN MDTDDERQYQ DFLEDLEEDE AIRKNVNIYR DSAIPVESDT DDEGAPRISL AEMLEDLHIS QDATGEEGAS MLT
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Molecular Weight
57.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NMD3 is a highly conserved protein that plays a crucial role in the nuclear export of ribosomal subunits in eukaryotic cells. It interacts specifically with nascent ribosomal proteins and the ribosomal RNA to facilitate their transport from the nucleus to the cytoplasm, where they participate in translation. The research surrounding NMD3 has gained momentum due to its significant implications in understanding ribosome biogenesis, a fundamental process essential for cell growth and function. Disruptions in ribosome assembly and export can lead to various cellular pathologies, including cancer and genetic disorders. Additionally, studying NMD3 provides insight into the molecular mechanisms of nucleocytoplasmic transport, a vital process for maintaining cellular homeostasis. Characterizing NMD3 through recombinant protein techniques has allowed researchers to elucidate its structural and functional properties, enabling the identification of its interaction partners and regulatory mechanisms. This research not only enhances our understanding of ribosome formation and export but also paves the way for the development of potential therapeutic strategies targeting ribosomal dysfunctions linked to disease. Overall, the study of NMD3 and its role in ribosome maturation is pivotal in the broader context of gene expression regulation and cellular health.
