Analytical Data
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Gene name
SAE1
- Application
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Alternative Names
SAE1;AOS1;SUA1;UBLE1A;SUMO-activating enzyme subunit 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBE0
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Expression Region
1-346aa
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AA Sequence
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAE IAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQ NLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNS IKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLD SSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFR TDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SAE1, or Sumoylation Activating Enzyme 1, plays a crucial role in the post-translational modification known as SUMOylation, which is vital for regulating various cellular processes including gene expression, DNA repair, and cellular stress responses. The interest in SAE1 has grown in recent years due to its involvement in numerous pathological conditions, such as cancer and neurodegenerative diseases. Understanding the structure and function of SAE1, especially in its reconstituted recombinant form, is essential for elucidating its mechanisms and potential as a therapeutic target. Research into SAE1 focuses on its enzymatic activity, interaction with substrates, and its regulation, which can provide insights into the broader SUMOylation pathway. The development of recombinant SAE1 allows for detailed biochemical and biophysical studies, paving the way for the identification of specific inhibitors or modulators that could influence SAE1 activity. By manipulating this pathway, scientists aim to uncover new strategies for disease intervention, highlighting SAE1 as a promising candidate in drug discovery and development.
