Analytical Data
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基因名
SAE1
- Application
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别名
SAE1;AOS1;SUA1;UBLE1A;SUMO-activating enzyme subunit 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UBE0
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表达区间
1-346aa
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氨基酸序列
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAE IAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQ NLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNS IKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLD SSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFR TDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SAE1, or Sumoylation Activating Enzyme 1, plays a crucial role in the post-translational modification known as SUMOylation, which is vital for regulating various cellular processes including gene expression, DNA repair, and cellular stress responses. The interest in SAE1 has grown in recent years due to its involvement in numerous pathological conditions, such as cancer and neurodegenerative diseases. Understanding the structure and function of SAE1, especially in its reconstituted recombinant form, is essential for elucidating its mechanisms and potential as a therapeutic target. Research into SAE1 focuses on its enzymatic activity, interaction with substrates, and its regulation, which can provide insights into the broader SUMOylation pathway. The development of recombinant SAE1 allows for detailed biochemical and biophysical studies, paving the way for the identification of specific inhibitors or modulators that could influence SAE1 activity. By manipulating this pathway, scientists aim to uncover new strategies for disease intervention, highlighting SAE1 as a promising candidate in drug discovery and development.
