Cat: PA1000-2771

Recombinant Human RPRD1A Protein,His

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Analytical Data

  • Gene name

    RPRD1A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    RPRD1A;P15RS;Regulation of nuclear pre-mRNA domain-containing Protein 1A

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q96P16

  • Expression Region

    1-312aa

  • AA Sequence

    MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED

  • Molecular Weight

    38 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

RPRD1A (Regulation of Pre-mRNA Processing Domain 1A) is a protein that plays a significant role in the regulation of gene expression, particularly in pre-mRNA splicing and transcriptional processes. It is part of a larger family of proteins involved in the coupling of transcription and mRNA processing, and its dysfunction has been linked to various diseases, including cancer. The protein interacts with several key components of the spliceosome, influencing the maturation of mRNA transcripts and their subsequent translation into proteins. Recent studies have focused on characterizing the molecular mechanisms by which RPRD1A regulates splicing and transcription termination, as well as its role in the cellular response to stress and other stimuli. Understanding the precise function of RPRD1A and its interactions within the spliceosome could provide insights into the intricate regulatory networks governing gene expression. This research is crucial for developing therapeutic strategies targeting aberrant splicing and gene regulation associated with various human diseases. Furthermore, RPRD1A has emerged as a potential biomarker for certain cancers, making it a target of interest for both diagnostic and therapeutic applications. Overall, the study of RPRD1A and its recombinant forms is vital for uncovering the complexities of mRNA processing and its implications for human health.

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