Analytical Data
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Gene name
NEK10
- Application
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Alternative Names
NEK10; Serine/threonine-protein kinase Nek10; EC 2.7.11.1; Never in mitosis A-related kinase 10; NimA-related protein kinase 10
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZWH5
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Expression Region
1-474 aa
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AA Sequence
MVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR
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Molecular Weight
79.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The NEK10 protein, a member of the NIMA-related kinase family, has garnered attention in recent years due to its potential implications in cell cycle regulation and various diseases, including cancer. NEK10 is involved in several critical cellular processes, such as mitosis and the DNA damage response, which positions it as a significant player in maintaining genomic stability. Research indicates that altered expression of NEK10 may contribute to tumorigenesis, highlighting its role as a potential biomarker or therapeutic target in cancer treatment. Additionally, studies have revealed that NEK10 may interact with other key proteins involved in cell signaling pathways, further implicating its importance in cellular physiology. Due to these associations, scientists are increasingly focused on studying NEK10's structure, function, and regulation. The recombinant production of NEK10 can enhance the understanding of its biochemical properties and interactions, facilitating the exploration of its role in disease and opening avenues for the development of novel therapeutic strategies. As research continues, the characterization of NEK10 through recombinant protein studies is expected to shed light on its mechanistic functions and contribution to human health, thereby advancing the field of molecular biology and cancer research.
