Analytical Data
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Gene name
rnhA
- Application
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Alternative Names
DHX9;DDX9;LKP;NDH2;ATP-dependent RNA helicase A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HSF6
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Expression Region
1-199aa
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AA Sequence
MPVVECDIQTARAALADAGASFSDGNSEHELWHADLGDAHAVAYADKLVVQGGSPTDITAVVQPDRGGRVHAYFDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDDIELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALANEALDDA
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on recombinant protein rnhA, which encodes RNase HI, has gained significant attention due to its essential role in various biological processes, particularly in RNA metabolism and DNA replication. RNase HI is an enzyme that specifically degrades the RNA strand of RNA-DNA hybrids, which is crucial for the removal of RNA primers during DNA replication, thereby ensuring genomic stability. Mutations or deficiencies in the rnhA gene can lead to a variety of cellular dysfunctions and diseases, making it a target for biomedical research. The production of recombinant rnhA allows for detailed studies of its enzymatic mechanisms, structural properties, and interactions with other cellular components. Moreover, understanding rnhA could have implications in developing therapeutic strategies against bacterial infections, as RNase HI is a potential drug target for antimicrobial agents. The advent of advanced expression systems and purification techniques has facilitated the efficient production and characterization of rnhA, enabling researchers to explore its functional roles and contributions to cellular homeostasis more comprehensively. This research not only enhances our understanding of fundamental cellular processes but also opens new avenues for biotechnology applications.
