Analytical Data
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基因名
USP2
- Application
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别名
USP2；UBP41；Ubiquitin carboxyl-terminal hydrolase 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75604
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表达区间
2-605aa
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氨基酸序列
MDYKDDDDKSQLSSTLKRYTESARYTDAHYAKSGYGAYTPSSYGANLAAS LLEKEKLGFKPVPTSSFLTRPRTYGPSSLLDYDRGRPLLRPDITGGGKRA ESQTRGTERPLGSGLSGGSGFPYGVTNNCLSYLPINAYDQGVTLTQKLDS QSDLARDFSSLRTSDSYRIDPRNLGRSPMLARTRKELCTLQGLYQTASCP EYLVDYLENYGRKGSASQVPSQAPPSRVPEIISPTYRPIGRYTLWETGKG QAPGPSRSSSPGRDGMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELR DYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQ IQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPD DEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLS LPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQ RFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYN LYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAY LLFYELASPPSRM
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USP2 (Ubiquitin-specific protease 2) is a member of the deubiquitinating enzyme family, which plays a crucial role in the regulation of cellular processes by removing ubiquitin moieties from target proteins. Ubiquitination is a post-translational modification that typically marks proteins for degradation via the proteasome, thus controlling protein levels and signaling pathways in the cell. Research into USP2 has gained momentum due to its involvement in various biological functions, including cell cycle regulation, DNA repair, and apoptosis, as well as its implications in cancer biology. Studies have shown that USP2 can stabilize several oncoproteins, such as MDM2 and cyclin D1, leading to enhanced tumorigenesis. Additionally, USP2 has been implicated in the modulation of immune responses and the pathogenesis of neurodegenerative diseases. The enzymatic activity and substrate specificity of USP2 make it a potential target for therapeutic intervention, particularly in cancer treatment, where its inhibition could restore the degradation of aberrant proteins. As a result, understanding the biochemical properties and regulatory mechanisms of USP2 through studies involving its recombinant protein is essential. Researchers have focused on expressing and purifying USP2 recombinant protein to elucidate its functions, interactions, and structural characteristics, providing valuable insights that could lead to the development of novel therapeutic strategies targeting this enzyme in various diseases.
