Analytical Data
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Gene name
PTPRC
- Application
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Alternative Names
PTPRC;CD45;Receptor-type tyrosine-Protein phosphatase C
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08575
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Expression Region
26-577aa
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AA Sequence
QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
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Molecular Weight
76.8kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PTPRC, also known as CD45, is a critical receptor-type protein tyrosine phosphatase predominantly expressed on the surface of leukocytes. Its role in modulating immune responses has made it a focal point in immunological research. PTPRC is vital for T cell activation and differentiation as it participates in the regulation of various signaling pathways, including those initiated by the T cell receptor. Dysregulation of PTPRC activity has been implicated in numerous autoimmune disorders, cancers, and infectious diseases, highlighting its potential as a therapeutic target. The recombinant expression of PTPRC in various systems facilitates the in-depth study of its biochemical properties and functional roles in immune regulation. Researchers employ techniques such as gene cloning and protein expression to produce purified PTPRC proteins, which are then used in binding assays, enzyme activity assays, and structural analyses. Understanding the intricacies of PTPRC's function at the molecular level can aid in elucidating the mechanisms behind immune system disorders and pave the way for novel treatment strategies. Furthermore, advances in biopharmaceutical technologies may enhance the development of PTPRC-modulating drugs, emphasizing the importance of continued research in this area to address prevalent health challenges associated with immune dysregulation.
