Analytical Data
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Gene name
OGC
- Application
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Alternative Names
OGC；SLC20A4；Mitochondrial 2-oxoglutarate/malate carrier Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q02978
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Expression Region
1-314aa
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AA Sequence
MAATASAGAGGIDGKPRTSPKSVKFLFGGLAGMGATVFVQPLDLVKNRMQ LSGEGAKTREYKTSFHALTSILKAEGLRGIYTGLSAGLLRQATYTTTRLG IYTVLFERLTGADGTPPGFLLKAVIGMTAGATGAFVGTPAEVALIRMTAD GRLPADQRRGYKNVFNALIRITREEGVLTLWRGCIPTMARAVVVNAAQLA SYSQSKQFLLDSGYFSDNILCHFCASMISGLVTTAASMPVDIAKTRIQNM RMIDGKPEYKNGLDVLFKVVRYEGFFSLWKGFTPYYARLGPHTVLTFIFL EQMNKAYKRLFLSG
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of OGC (ornithine transcarbamylase) recombinant proteins is pivotal in understanding metabolic disorders, particularly those associated with urea cycle deficiencies. OGC plays a crucial role in the conversion of ornithine and carbamoyl phosphate into citrulline, a key step in the urea cycle that detoxifies ammonia in the liver. Mutations in the OTC gene, which encodes OGC, lead to hyperammonemia and can result in severe neurological damage or even mortality if left untreated. Recombinant OGC proteins are vital for developing novel therapeutic strategies, including enzyme replacement therapies and gene therapy approaches, aimed at correcting the underlying genetic deficiencies. Furthermore, producing OGC in a recombinant form allows for detailed structural and functional studies, which facilitate the design of small molecules or substrates that can enhance its activity or stability. Thus, research involving OGC recombinant proteins not only advances our understanding of urea cycle disorders but also opens avenues for innovative treatments, ultimately improving patient outcomes in conditions stemming from urea cycle dysfunction.
