Analytical Data
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基因名
PRPS1
- Application
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别名
PRPS1;Ribose-phosphate pyrophosphokinase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P60891
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表达区间
2-318aa
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氨基酸序列
PNIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRLNVDFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL
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分子量
35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Identification
Protein Description
PRPS1 (Phosphoribosyl pyrophosphate synthetase 1) is a critical enzyme involved in the de novo synthesis of purine nucleotides, playing a vital role in cellular metabolism and nucleotide homeostasis. Mutations in the PRPS1 gene are associated with a range of clinical disorders, including X-linked nonsyndromic intellectual disability and other metabolic diseases, underscoring its importance in human health. The study of PRPS1 recombinant proteins has gained significant interest as it enables researchers to explore the enzyme's structure-function relationship, mechanisms of action, and interaction with various substrates. By generating recombinant PRPS1, scientists can investigate the biochemical properties of the enzyme in vitro, assess the impact of specific mutations, and evaluate potential therapeutic interventions for associated disorders. Furthermore, recombinant PRPS1 serves as a valuable tool for high-throughput screening of small molecule inhibitors that could modulate its activity, providing insights into novel strategies for treating diseases linked to purine metabolism dysregulation. Thus, research on PRPS1 recombinant proteins not only enhances our understanding of fundamental biological processes but also opens avenues for developing targeted therapies for genetic and metabolic conditions related to this enzyme.
