Analytical Data
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基因名
pknF
- Application
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别名
pknF；Serine/threonine-Protein kinase PknF
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P9WI74
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表达区间
1-476aa
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氨基酸序列
MPLAEGSTFAGFTIVRQLGSGGMGEVYLARHPRLPRQDALKVLRADVSADGEYRARFNREADAAASLWHPHIVAVHDRGEFDGQLWIDMDFVDGTDTVSLLRDRYPNGMPGPEVTEIITAVAEALDYAHERRLLHRDVKPANILIANPDSPDRRIMLADFGIAGWVDDPSGLTATNMTVGTVSYAAPEQLMGNELDGRADQYALAATAFHLLTGSPPFQHANPAVVISQHLSASPPAIGDRVPELTPLDPVFAKALAKQPKDRYQRCVDFARALGHRLGGAGDPDDTRVSQPVAVAAPAKRSLLRTAVIVPAVLAMLLVMAVAVAVREFQRADDERAAQPARTRTTTSAGTTTSVAPASTTRPAPTTPTTTGAADTATASPTAAVVAIGALCFPLGSTGTTKTGATAYCSTLQGTNTTIWSLTEDTVASPTVTATADPTEAPLPIEQESPIRVCMQQTGQTRRECREEIRRSNGWP
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分子量
78.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of pknF recombinants has garnered significant attention due to its implications in the field of microbial pathogenesis and antibiotic resistance. pknF is a gene found in mycobacterial species, particularly in Mycobacterium tuberculosis, the causative agent of tuberculosis, a disease that poses a major global health threat. This gene encodes for a serine/threonine protein kinase, which plays a vital role in various cellular processes, including signal transduction, cell growth, and stress response. Given its pivotal function, understanding the mechanisms regulating pknF activity and its interactions with other cellular components is essential for elucidating the virulence of mycobacteria and the development of potential therapeutic targets. Moreover, research on pknF recombinant proteins can provide valuable insights into the biochemical pathways involved in mycobacterial infection and survival within host cells. As antibiotic resistance continues to rise, exploring the role of pknF and its recombinant forms could reveal novel approaches for drug discovery, potentially leading to the development of innovative treatments for tuberculosis and other mycobacterial diseases. Consequently, this area of research not only enhances our understanding of microbial genetics and physiology but also contributes to the ongoing efforts to combat infectious diseases that remain a significant public health challenge worldwide.
