Analytical Data
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Gene name
MRPL14
- Application
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Alternative Names
1110006I11Rik; 39S ribosomal protein L14; 39S ribosomal protein L14 mitochondrial; 39S ribosomal protein L32; AI846816; L14mt; L32mt; mitochondrial; MRP L14; MRP L32; MRP-L14; MRP-L32; mrpl14; RM14_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P1L8
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Expression Region
31-145 aa
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AA Sequence
AIQKMTRVRV VDNSALGNSP YHRAPRCIHV YKKNGVGKVG DQILLAIKGQ KKKALIVGHC MPGPRMTPRF DSNNVVLIED NGNPVGTRIK TPIPTSLRKR EGEYSKVLAI AQNFV
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Molecular Weight
15.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MRPL14, or Mitochondrial Ribosomal Protein L14, is a vital component of the mitochondrial ribosome, playing a crucial role in protein synthesis within the mitochondria. Dysfunction of MRPL14 has been associated with various mitochondrial diseases, which can lead to severe metabolic disorders and mitochondrial dysfunction. Given that mitochondria are essential for cellular energy production and play significant roles in apoptosis and other cellular processes, understanding the function and structure of MRPL14 is paramount for elucidating its role in health and disease. Recent advances in molecular biology and recombinant protein technology have enabled the production of MRPL14 as a recombinant protein, facilitating the examination of its biochemical properties and interaction with other mitochondrial components. Research into MRPL14 also holds potential for therapeutic avenues, as targeting mitochondrial dysfunction is a promising strategy for treating diseases linked to mitochondrial anomalies. By studying MRPL14 in detail, researchers aim to uncover insights into mitochondrial biogenesis, regulation, and the implications of its dysfunction, ultimately contributing to the broader understanding of mitochondrial pathophysiology and potential intervention strategies.
