Analytical Data
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Gene name
PPP1R8
- Application
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Alternative Names
PPP1R8;ARD1;NIPP1;Nuclear inhibitor of Protein phosphatase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12972
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Expression Region
1-351aa
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AA Sequence
MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLGLEESG SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL IVEHHHHHH
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPP1R8, also known as Protein Phosphatase 1 Regulatory Subunit 8, plays a crucial role in regulating protein phosphatase 1 (PP1), an enzyme vital for various cellular processes, including cell division, metabolism, and signal transduction. Research has shown that PPP1R8 interacts with PP1 to modulate its activity, particularly in the context of specific signaling pathways involved in cellular responses. Dysregulation of this interaction has been implicated in several diseases, including cancer and neurodegenerative disorders, making PPP1R8 an attractive target for therapeutic interventions. The study of recombinant PPP1R8 has become increasingly important for understanding its structure-function relationship and the mechanisms by which it regulates PP1 activity. By generating and characterizing recombinant forms of PPP1R8, researchers can elucidate its role in cellular signaling and explore potential applications in drug development. Additionally, the expression and purification of this protein enable the investigation of its interactions with various substrates and regulators, providing insights into its functional dynamics and its potential impact on disease processes. Thus, the study of PPP1R8 not only broadens our understanding of PP1 regulation but also paves the way for innovative strategies in treating conditions associated with its dysregulation.
