Analytical Data
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基因名
PPIE
- Application
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别名
PPIE;CYP33;Peptidyl-prolyl cis-trans isomerase E
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UNP9
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表达区间
1-301aa
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氨基酸序列
MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD WLKKFSGKTL EENKEEEGSE PPKAETQEGE PIAKKARSNP QVYMDIKIGN KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVIIADCGEY V
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The PPIE (Peptidyl-Prolyl Isomerase E) protein plays a crucial role in various cellular processes, including cell signaling, protein folding, and stress responses. Recent research has shown that PPIE is involved in critical biological functions, such as cell cycle regulation and immune response modulation. Dysregulation of PPIE has been linked to various diseases, including cancer and neurodegenerative disorders. Given the increasing awareness of its significance, scientists are focusing on understanding the molecular mechanisms underlying its function. Studies involving the reconstituted form of PPIE have enabled researchers to investigate its structure-function relationship more thoroughly. This includes exploring how alterations in its conformation can affect cellular pathways and influence disease progression. The development of recombinant PPIE proteins has allowed for detailed biochemical assays, high-throughput screening, and potential therapeutic applications. Moreover, the integration of advanced technologies such as cryo-electron microscopy and nuclear magnetic resonance has provided deeper insights into the protein's dynamics. By elucidating the role of PPIE in cellular homeostasis and disease, researchers aim to identify novel therapeutic targets and strategies to combat associated pathologies. Overall, the study of PPIE and its reconstituted protein forms represents a promising frontier in molecular biology, with significant implications for understanding and treating various human diseases.












