Cat: PA2000-9390

Recombinant Human MLX Protein,GST

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Analytical Data

  • Gene name

    MLX

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    MLXIP; BHLHE36; KIAA0867; MIR; MONDOA; MLX-interacting protein; Class E basic helix-loop-helix protein 36; bHLHe36; Transcriptional activator MondoA

  • Species

    Human

  • Source

    E. coli

  • Tag

    GST-tag at N-terminal

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9HAP2

  • Expression Region

    1-244 aa

  • AA Sequence

    MTEPGASPEDPWVKVEYAYSDNSLDPGLFVESTRKGSVVSRANSIGSTSASSVPNTDDEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVLHQLKNQLY

  • Molecular Weight

    52.58 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

MLX, a member of the basic helix-loop-helix (bHLH) transcription factor family, plays a crucial role in regulating various biological processes, including cellular metabolism, proliferation, and differentiation. Recent research has highlighted MLX as a key integrator of metabolic signals, linking nutrient availability to gene expression. Its ability to form heterodimers with other bHLH proteins, such as MondoA, enhances its regulatory capabilities in response to glucose and fatty acid levels. Furthermore, MLX has been implicated in the development of various metabolic disorders, including obesity and diabetes, making it an attractive target for therapeutic intervention. The study of MLX has gained momentum as advanced techniques in molecular biology and biochemistry allow for a deeper understanding of its structural and functional properties. By exploring the mechanisms underlying MLX’s activities, researchers aim to identify potential strategies for modulating its function, potentially leading to novel treatments for metabolic diseases. The continued investigation of MLX and its associated proteins is essential for unraveling the complexities of metabolic regulation and developing innovative approaches to address related health issues.

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