Analytical Data
-
基因名
POLR2K
- Application
-
别名
POLR2K;DNA-directed RNA polymerases I. II. and III subunit RPABC4
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P53803
-
表达区间
1-58aa
-
氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMDTQKDVQPPKQQPMIYICGECHTENE IKSRDPIRCRECGYRIMYKKRTKRLVVFDAR
-
分子量
9 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human MLXIPL Protein，His
Cat：PA2000-9389
Recombinant Human PLCb4 Protein，His
Cat：PA2000-862DB
Protein Description
POLR2K, one of the essential components of RNA polymerase II, plays a crucial role in the transcription of protein-coding genes in eukaryotic cells. As part of the core enzyme, POLR2K is involved in the synthesis of messenger RNA (mRNA), which is pivotal for translating genetic information into proteins. Recent studies have highlighted the significance of POLR2K in various pathological conditions, including cancer, where its overexpression has been linked to enhanced cell proliferation and tumorigenesis. Additionally, POLR2K is critical for the regulation of gene expression and response to environmental stimuli, making it a key player in developmental processes and cellular differentiation. Recombinant POLR2K proteins have emerged as valuable tools for studying the mechanisms of transcription and gene regulation, as well as for potential therapeutic applications. Researchers are focusing on the structural and functional characterization of POLR2K to understand its interactions with other transcription factors and regulatory elements within the transcription machinery. This research aims to delineate the pathways influenced by POLR2K and explore its implications in health and disease, ultimately contributing to the development of targeted therapies that may mitigate the aberrant transcriptional processes associated with various disorders.
