Analytical Data
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Gene name
MLST8
- Application
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Alternative Names
G protein beta subunit like; G protein beta subunit-like; Gable; GbetaL; GBL; GBL protein; LST8; LST8_HUMAN; Mammalian lethal with SEC13 protein 8; MGC111011; mLST8; MTOR associated protein LST8 homolog (S. cerevisiae); POP3; POP3 homolog (S. pombe); Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1; WAT1 homolog (S. pombe)
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BVC4
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Expression Region
1-326 aa
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AA Sequence
MNTSPGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNALEV TPDRSMIAAA GYQHIRMYDL NSNNPNPIIS YDGVNKNIAS VGFHEDGRWM YTGGEDCTAR IWDLRSRNLQ CQRIFQVNAP INCVCLHPNQ AELIVGDQSG AIHIWDLKTD HNEQLIPEPE VSITSAHIDP DASYMAAVNS TGNCYVWNLT GGIGDEVTQL IPKTKIPAHT RYALQCRFSP DSTLLATCSA DQTCKIWRTS NFSLMTELSI KSGNPGESSR GWMWGCAFSG DSQYIVTASS DNLARLWCVE TGEIKREYGG HQKAVVCLAF NDSVLG
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Molecular Weight
35.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MLST8, also known as GbetaL, is a critical component of the mTOR (mechanistic target of rapamycin) signaling pathway, which is essential for regulating cell growth, proliferation, and metabolism. Research into the recombinant protein MLST8 has gained significant attention due to its pivotal role in various biological processes and its implications in cancer and metabolic diseases. The mTOR pathway, which MLST8 is a part of, integrates signals from nutrients, growth factors, and energy status, thereby influencing cellular responses to environmental changes. Abnormalities in this signaling pathway, often involving MLST8, can lead to uncontrolled cell proliferation and tumorigenesis. Therefore, understanding the structure and function of MLST8 as a recombinant protein can provide insights into its mechanisms of action and potential therapeutic targets. Furthermore, recombinant MLST8 can also be utilized to explore its interactions with other mTOR complex components, enhancing our comprehension of the entire signaling cascade. This research is crucial for developing novel strategies for treating diseases associated with dysregulated mTOR signaling, making MLST8 an important focus in molecular biology and biomedical research.
