Analytical Data
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Gene name
POLR2J2
- Application
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Alternative Names
SATB1;DNA-binding Protein SATB1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9GZM3
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Expression Region
1-115aa
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AA Sequence
MNAPPAFESF LLFEGEKITI NKDTKVPNAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLR2J2, part of the eukaryotic RNA polymerase II complex, plays a crucial role in the transcription process, facilitating the synthesis of messenger RNA (mRNA) from DNA templates. As a component of the core polymerase structure, POLR2J2 contributes to the regulation of gene expression, making it essential for cellular function and development. Studies have shown that variations in POLR2J2 expression levels can impact various biological processes, including cell differentiation and response to stress. Research focusing on the recombinant expression of POLR2J2 aims to elucidate its functional dynamics and interactions within the transcription machinery. By using techniques such as gene cloning and protein purification, scientists strive to generate large quantities of the POLR2J2 protein, enabling in-depth biochemical assays and structural analysis. Understanding the specific roles and mechanisms of POLR2J2 can provide insights into transcription regulation and its implications in diseases, particularly those linked to aberrant gene expression. Overall, the study of POLR2J2 recombinant protein is a vital step toward unraveling the complexities of transcriptional control in eukaryotic cells.
