Analytical Data
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Gene name
HMGCS2
- Application
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Alternative Names
HMGCS2；Hydroxymethylglutaryl-CoA synthase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54868
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Expression Region
38-508aa
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AA Sequence
TASAVPLAKTDTWPKDVGILALEVYFPAQYVDQTDLEKYNNVEAGKYTVGLGQTRMGFCSVQEDINSLCLTVVQRLMERIQLPWDSVGRLEVGTETIIDKSKAVKTVLMELFQDSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGRYAMVVCGDIAVYPSGNARPTGGAGAVAMLIGPKAPLALERGLRGTHMENVYDFYKPNLASEYPIVDGKLSIQCYLRALDRCYTSYRKKIQNQWKQAGSDRPFTLDDLQYMIFHTPFCKMVQKSLARLMFNDFLSASSDTQTSLYKGLEAFGGLKLEDTYTNKDLDKALLKASQDMFDKKTKASLYLSTHNGNMYTSSLYGCLASLLSHHSAQELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV
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Molecular Weight
59.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HMGCS2, or 3-hydroxy-3-methylglutaryl-CoA synthase 2, plays a critical role in ketogenesis in the liver and is essential for energy metabolism, particularly during fasting or prolonged starvation. It catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA to form HMG-CoA, a key precursor in the synthesis of ketone bodies. Recent studies have highlighted the physiological importance of HMGCS2 in metabolic disorders, such as obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD), as its enzyme activity is believed to influence lipid metabolism and ketone body production. Increased interest in HMGCS2 has led researchers to investigate its potential as a therapeutic target for enhancing ketogenesis and improving metabolic health. The recombinant protein of HMGCS2 is particularly valuable for these studies, providing a model for enzyme kinetics and allowing for high-throughput screening of potential inhibitors or enhancers of its activity. Furthermore, understanding the structure-function relationship of this enzyme could aid in the design of strategies to modulate its activity in metabolic diseases. Overall, the study of HMGCS2 recombinant protein opens new avenues for metabolic research and therapeutic interventions aimed at regulating energy homeostasis and addressing metabolic disorders.
