Analytical Data
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Gene name
ABCG5
- Application
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Alternative Names
ABCG5；ATP-binding cassette sub-family G member 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H222
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Expression Region
1-651aa
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AA Sequence
MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLGRAGTFLGEVYVNGRALRREQFQDCFSYVLQSDTLLSSLTVRETLHYTALLAIRRGNPGSFQKKVEAVMAELSLSHVADRLIGNYSLGGISTGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLVELARRNRIVVLTIHQPRSELFQLFDKIAILSFGELIFCGTPAEMLDFFNDCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETSKRVQMIESAYKKSAICHKTLKNIERMKHLKTLPMVPFKTKDSPGVFSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRSNVLKGAIQDRVGLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSSNVSVTTNPMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLISR
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Molecular Weight
72.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABCG5 is a member of the ATP-binding cassette (ABC) transporter family, which plays a crucial role in cholesterol and phytosterol homeostasis in humans and other organisms. Mutations or defects in the ABCG5 gene are associated with sitosterolemia, a rare genetic disorder characterized by increased absorption of plant sterols and cholesterol, leading to premature atherosclerosis. The protein functions primarily in the liver and intestine, mediating the efflux of excess sterols from cells into the bile and the intestinal lumen, respectively. Research on recombinant ABCG5 protein has gained traction to better understand its molecular mechanisms, facilitating the design of targeted therapies for related disorders. By producing and characterizing the protein in a laboratory setting, scientists aim to elucidate its structural, functional, and interaction properties, shedding light on its role in lipid metabolism. Investigations into ABCG5 not only enhance our comprehension of sterol regulation but also pave the way for developing pharmacological interventions to manage lipid disorders linked to this transporter. The study of recombinant ABCG5 is crucial for unraveling the complexities of cholesterol metabolism and addressing the cardiovascular risks associated with dysregulated sterol levels.
