Analytical Data
-
Gene name
POLE3
- Application
-
Alternative Names
POLE3;CHRAC17;DNA polymerase epsilon subunit 3
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9NRF9
-
Expression Region
1-147aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAERPED LNLPNAVITR IIKEALPDGV NISKEARSAI SRAASVFVLY ATSCANNFAM KGKRKTLNAS DVLSAMEEME FQRFVTPLKE ALEAYRREQK GKKEASEQKK KDKDKKTDSE EQDKSRDEDN DEDEERLEEE EQNEEEEVDN
-
Molecular Weight
19 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLE3, a member of the polymerase epsilon complex, is an essential protein involved in eukaryotic DNA replication and repair. Recent studies have revealed that POLE3 plays a critical role in maintaining genomic stability, as it is integral to the proofreading activity during DNA synthesis, helping to correct errors that may arise during replication. Given its importance, researchers have begun to explore the implications of POLE3 dysregulation in various diseases, particularly cancer, where mutations in DNA polymerases can lead to increased mutation rates and tumorigenesis. The recombinant expression of POLE3 has become a focal point for understanding its functional mechanisms and potential therapeutic roles. By studying POLE3 in a controlled environment, scientists aim to elucidate its interaction with other replication factors and its contribution to high-fidelity DNA synthesis. Additionally, the development of POLE3 as a biomarker or therapeutic target is being investigated, as alterations in its expression may correlate with specific cancer phenotypes and could provide insights into tumor biology. Overall, the research surrounding recombinant POLE3 not only enhances our understanding of DNA replication processes but also opens avenues for innovative cancer treatment strategies by targeting the pathways influenced by this crucial polymerase component.
