Analytical Data
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Gene name
MKRN2
- Application
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Alternative Names
MKRN2; RNF62; HSPC070Probable E3 ubiquitin-protein ligase makorin-2; EC 2.3.2.27; RING finger protein 62; RING-type E3 ubiquitin transferase makorin-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H000
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Expression Region
1-416 aa
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AA Sequence
MSTKQITCRY FMHGVCREGS QCLFSHDLAN SKPSTICKYY QKGYCAYGTR CRYDHTRPSA AAGGAVGTMA HSVPSPAFHS PHPPSEVTAS IVKTNSHEPG KREKRTLVLR DRNLSGMAER KTQPSMVSNP GSCSDPQPSP EMKPHSYLDA IRSGLDDVEA SSSYSNEQQL CPYAAAGECR FGDACVYLHG EVCEICRLQV LHPFDPEQRK AHEKICMLTF EHEMEKAFAF QASQDKVCSI CMEVILEKAS ASERRFGILS NCNHTYCLSC IRQWRCAKQF ENPIIKSCPE CRVISEFVIP SVYWVEDQNK KNELIEAFKQ GMGKKACKYF EQGKGTCPFG SKCLYRHAYP DGRLAEPEKP RKQLSSQGTV RFFNSVRLWD FIENRESRHV PNNEDVDMTE LGDLFMHLSG VESSEP
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Molecular Weight
46.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MKRN2 (Makorin Ring Finger Protein 2) is an E3 ubiquitin ligase that plays a critical role in various cellular processes, including protein degradation, cellular signaling, and regulation of gene expression. This protein has garnered attention due to its involvement in important biological pathways and potential implications in various diseases, particularly cancer. Research indicates that MKRN2 may be implicated in the regulation of key oncogenic pathways, suggesting that it could serve as a biomarker or therapeutic target in oncology. Furthermore, studies have shown that MKRN2 can influence the stability and activity of several proteins involved in cell cycle regulation and apoptosis. The development of recombinant MKRN2 protein is essential for elucidating its function and mechanism of action in a cellular context. By producing the MKRN2 protein in a controlled laboratory setting, researchers can perform biochemical assays, structural analysis, and functional studies to better understand its role in cellular processes and disease mechanisms. Additionally, recombinant MKRN2 could be instrumental in exploring its interactions with other proteins and potential involvement in post-translational modifications. Overall, the study of MKRN2 and its recombinant form represents a promising avenue for uncovering new insights into cellular regulation and could lead to novel therapeutic strategies for diseases associated with its dysregulation.
