Analytical Data
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Gene name
POLD4
- Application
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Alternative Names
POLD4;POLDS;DNA polymerase delta subunit 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HCU8
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Expression Region
1-107aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL
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Molecular Weight
15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLD4, or DNA polymerase delta 4, is a crucial component of the DNA replication machinery, playing a significant role in the repair and replication of DNA. This polymerase, part of the DNA polymerase delta family, is particularly important for maintaining genomic stability, especially during the S-phase of the cell cycle. Recent studies indicate that POLD4 is involved in damage tolerance pathways, allowing cells to survive and replicate under genotoxic stress. Its overexpression has been linked to various cancers, suggesting its potential as a biomarker for tumor progression and a target for therapeutic interventions. The functional characterization of recombinant POLD4 proteins provides insights into their enzymatic properties, such as processivity and fidelity during DNA synthesis, which are essential for understanding its role in cellular processes. Furthermore, studying the recombinant protein enables the exploration of potential inhibitors or modulators that could disrupt aberrant polymerase activity in cancer cells. The ongoing research on POLD4 not only enhances our understanding of DNA polymerase dynamics but also contributes to the development of novel cancer treatment strategies, emphasizing its significance in molecular biology and therapeutic applications.
