Analytical Data
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基因名
PNP
- Application
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别名
PNP;NP;Purine nucleoside phosphorylase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00491
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表达区间
1-289aa
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氨基酸序列
MENGYTYEDYKNTAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYGEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASIPLPDKAS
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分子量
48.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNP (Purine Nucleoside Phosphorylase) is an essential enzyme in the purine salvage pathway, playing a critical role in nucleotide metabolism by catalyzing the phosphorolysis of purine nucleosides to their corresponding bases and ribose-1-phosphate. Dysfunction of PNP can lead to severe immunodeficiency disorders, highlighting its importance in cellular physiology and immune function. Research on PNP recombinant proteins has gained momentum as scientists seek to elucidate its structure-function relationships, interaction with various ligands, and regulatory mechanisms. The ability to produce PNP in a recombinant form allows for detailed biochemical characterization and the development of potential therapeutic strategies, including enzyme replacement therapy for genetic deficiencies. Additionally, understanding the enzymatic activity of PNP and its implications in various pathological conditions, such as lymphoproliferative disorders and certain cancers, provides insights into its potential as a therapeutic target. Advances in protein expression systems, purification techniques, and structural biology have facilitated the comprehensive study of PNP, paving the way for innovative applications in drug development and medical research.
