Analytical Data
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Gene name
MTIF2
- Application
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Alternative Names
MTIF2；Translation initiation factor IF-2. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46199
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Expression Region
30-727aa
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AA Sequence
L RQWRHGFSSA YPVWTAQLCA WPWPTDVLTG AALSQYRLLV TKKEEGPWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKVR KNKDAVRRPQ ADPALLTPRS PVVTIMGHVD HGKTTLLDKF RKTQVAAVET GGITQHIGAF LVSLPSGEKI TFLDTPGHAA FSAMRARGAQ VTDIVVLVVA ADDGVMKQTV ESIQHAKDAQ VPIILAVNKC DKAEADPEKV KKELLAYDVV CEDYGGDVQA VPVSALTGDN LMALAEATVA LAEMLELKAD PNGPVEGTVI ESFTDKGRGL VTTAIIQRGT LRKGSVLVAG KCWAKVRLMF DENGKTIDEA YPSMPVGITG WRDLPSAGEE ILEVESEPRA REVVDWRKYE QEQEKGQEDL KIIEEKRKEH KEAHQKAREK YGHLLWKKRS ILRFLERKEQ IPLKPKEKRE RDSNVLSVII KGDVDGSVEA ILNIIDTYDA SHECELELVH FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLHKIIY RLVEDLQEEL SSRLPCAVEE HPVGEASILA TFSVTEGKKK VPVAGCRVQK GQLEKQKKFK LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN MEFQVGDRIV CYEEKQIQAK TSWDPGF
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Molecular Weight
81.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MTIF2, or Mitochondrial Translation Initiation Factor 2, plays a crucial role in the mitochondrial protein synthesis process. Unlike the well-studied cytosolic translation factors, the understanding of mitochondrial translation mechanisms remains limited, making MTIF2 a significant focus in mitochondrial biology. Research has shown that MTIF2 is essential for the initiation of mitochondrial protein translation, particularly for the synthesis of polypeptides encoded by mitochondrial DNA that are critical for respiratory chain function. Deficiencies in MTIF2 or its associated pathways have been linked to various mitochondrial disorders, highlighting its potential as a biomarker for mitochondrial dysfunction. Moreover, as mitochondrial diseases often have complex, multifactorial origins, understanding the precise function and regulation of MTIF2 offers insights into potential therapeutic strategies. Recent advances in structural biology have provided a clearer picture of how MTIF2 interacts with mitochondrial ribosomes and other translational components, facilitating the understanding of its role in mitochondrial function. Furthermore, elucidating the mechanism by which MTIF2 influences mitochondrial translation can pave the way for novel interventions aimed at treating mitochondrial conditions. Overall, research on MTIF2 is pivotal not only for deciphering mitochondrial protein synthesis but also for developing targeted therapies for related pathologies.
