Analytical Data
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基因名
MTIF2
- Application
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别名
MTIF2；Translation initiation factor IF-2. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P46199
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表达区间
30-727aa
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氨基酸序列
L RQWRHGFSSA YPVWTAQLCA WPWPTDVLTG AALSQYRLLV TKKEEGPWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKVR KNKDAVRRPQ ADPALLTPRS PVVTIMGHVD HGKTTLLDKF RKTQVAAVET GGITQHIGAF LVSLPSGEKI TFLDTPGHAA FSAMRARGAQ VTDIVVLVVA ADDGVMKQTV ESIQHAKDAQ VPIILAVNKC DKAEADPEKV KKELLAYDVV CEDYGGDVQA VPVSALTGDN LMALAEATVA LAEMLELKAD PNGPVEGTVI ESFTDKGRGL VTTAIIQRGT LRKGSVLVAG KCWAKVRLMF DENGKTIDEA YPSMPVGITG WRDLPSAGEE ILEVESEPRA REVVDWRKYE QEQEKGQEDL KIIEEKRKEH KEAHQKAREK YGHLLWKKRS ILRFLERKEQ IPLKPKEKRE RDSNVLSVII KGDVDGSVEA ILNIIDTYDA SHECELELVH FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLHKIIY RLVEDLQEEL SSRLPCAVEE HPVGEASILA TFSVTEGKKK VPVAGCRVQK GQLEKQKKFK LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN MEFQVGDRIV CYEEKQIQAK TSWDPGF
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分子量
81.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MTIF2, or Mitochondrial Translation Initiation Factor 2, plays a crucial role in the mitochondrial protein synthesis process. Unlike the well-studied cytosolic translation factors, the understanding of mitochondrial translation mechanisms remains limited, making MTIF2 a significant focus in mitochondrial biology. Research has shown that MTIF2 is essential for the initiation of mitochondrial protein translation, particularly for the synthesis of polypeptides encoded by mitochondrial DNA that are critical for respiratory chain function. Deficiencies in MTIF2 or its associated pathways have been linked to various mitochondrial disorders, highlighting its potential as a biomarker for mitochondrial dysfunction. Moreover, as mitochondrial diseases often have complex, multifactorial origins, understanding the precise function and regulation of MTIF2 offers insights into potential therapeutic strategies. Recent advances in structural biology have provided a clearer picture of how MTIF2 interacts with mitochondrial ribosomes and other translational components, facilitating the understanding of its role in mitochondrial function. Furthermore, elucidating the mechanism by which MTIF2 influences mitochondrial translation can pave the way for novel interventions aimed at treating mitochondrial conditions. Overall, research on MTIF2 is pivotal not only for deciphering mitochondrial protein synthesis but also for developing targeted therapies for related pathologies.
