Analytical Data
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Gene name
MIR16
- Application
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Alternative Names
Glycerophosphodiester phosphodiesterase 1. Glycerophosphoinositol glycerophosphodiesterase GDE1. EC:3.1.4.44. Lysophospholipase D GDE1. EC:3.1.4.-. Membrane-interacting protein of RGS16. RGS16-interacting membrane protein
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NZC3
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Expression Region
1-331 aa
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AA Sequence
MWLWEDQGGLLGPFSFLLLVLLLVTRSPVNACLLTGSLFVLLRVFSFEPVPSCRALQVLKPRDRISAIAHRGGSHDAPENTLAAIRQAAKNGATGVELDIEFTSDGIPVLMHDNTVDRTTDGTGRLCDLTFEQIRKLNPAANHRLRNDFPDEKIPTLREAVAECLNHNLTIFFDVKGHAHKATEALKKMYMEFPQLYNNSVVCSFLPEVIYKMRQTDRDVITALTHRPWSLSHTGDGKPRYDTFWKHFIFVMMDILLDWSMHNILWYLCGISAFLMQKDFVSPAYLKKWSAKGIQVVGWTVNTFDEKSYYESHLGSSYITDSMVEDCEPHF
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Molecular Weight
64.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MIR16 recombinant protein has garnered significant interest in the field of molecular biology and biotechnology due to its potential applications in medicine and research. MIR16, a microRNA, plays a critical role in the regulation of gene expression and is implicated in various physiological processes, including cell proliferation, apoptosis, and differentiation. Its dysregulation has been associated with several diseases, particularly cancer. Understanding the functional mechanisms of MIR16 is crucial for developing novel therapeutic strategies aimed at modulating its activity for clinical applications. Researchers have been focusing on producing MIR16 recombinant proteins to study their interactions with target mRNAs and proteins, thereby elucidating their role in cancer biology and other diseases. The successful expression and purification of MIR16 in suitable host systems, such as bacterial or eukaryotic cells, can facilitate in vitro and in vivo studies, paving the way for targeted gene therapies and RNA-based treatments. Furthermore, the recombinant MIR16 can serve as a valuable tool for high-throughput screenings and biotechnological applications, allowing researchers to manipulate cellular pathways involved in disease progression. Overall, the investigation of MIR16 recombinant proteins holds promise for improving our understanding of microRNA biology and developing innovative approaches for disease intervention.
