Analytical Data
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Gene name
ABAT
- Application
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Alternative Names
ABAT；GABAT；4-aminobutyrate aminotransferase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P80404
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Expression Region
29-500aa
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AA Sequence
SQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFVEKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPADVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLLDLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLNIFSDILADFK
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Molecular Weight
79.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ABAT (4-aminobutyrate aminotransferase) is an enzyme that plays a crucial role in the metabolism of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. Research on ABAT has gained attention due to its involvement in various neurological disorders, including epilepsy, schizophrenia, and anxiety disorders. Understanding the function and regulation of ABAT is essential for elucidating the biochemical pathways that maintain GABA homeostasis in the brain. The recombinant expression of ABAT has been a pivotal aspect of recent studies, allowing for the characterization of the enzyme's kinetics, substrate specificity, and interaction with other molecular partners. Through the use of techniques such as site-directed mutagenesis and structural biology approaches, researchers aim to identify critical residues that influence the enzyme's activity and to determine how variations in ABAT can contribute to individual differences in neurotransmitter levels and susceptibility to psychiatric conditions. Moreover, recombinant ABAT could serve as a potential therapeutic target, paving the way for the development of novel drugs aimed at modulating GABAergic signaling. Overall, the study of ABAT and its recombinant protein offers significant insights into the biochemical mechanisms underlying mental health disorders, providing a foundational basis for future therapeutic strategies.
