Analytical Data
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基因名
ABAT
- Application
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别名
ABAT；GABAT；4-aminobutyrate aminotransferase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P80404
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表达区间
29-500aa
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氨基酸序列
SQAAAKVDVEFDYDGPLMKTEVPGPRSQELMKQLNIIQNAEAVHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFVEKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPADVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLLDLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLNIFSDILADFK
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分子量
79.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ABAT (4-aminobutyrate aminotransferase) is an enzyme that plays a crucial role in the metabolism of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. Research on ABAT has gained attention due to its involvement in various neurological disorders, including epilepsy, schizophrenia, and anxiety disorders. Understanding the function and regulation of ABAT is essential for elucidating the biochemical pathways that maintain GABA homeostasis in the brain. The recombinant expression of ABAT has been a pivotal aspect of recent studies, allowing for the characterization of the enzyme's kinetics, substrate specificity, and interaction with other molecular partners. Through the use of techniques such as site-directed mutagenesis and structural biology approaches, researchers aim to identify critical residues that influence the enzyme's activity and to determine how variations in ABAT can contribute to individual differences in neurotransmitter levels and susceptibility to psychiatric conditions. Moreover, recombinant ABAT could serve as a potential therapeutic target, paving the way for the development of novel drugs aimed at modulating GABAergic signaling. Overall, the study of ABAT and its recombinant protein offers significant insights into the biochemical mechanisms underlying mental health disorders, providing a foundational basis for future therapeutic strategies.
