Analytical Data
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Gene name
PHOSPHO1
- Application
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Alternative Names
PHOSPHO1;Phosphoethanolamine/phosphocholine phosphatase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCT1
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Expression Region
1-267aa
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AA Sequence
MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC
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Molecular Weight
33.7kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PHOSPHO1 is a phosphatase that plays a crucial role in skeletal mineralization and bone turnover. Its significance is underscored by its involvement in the regulatory mechanisms that govern phosphate and pyrophosphate levels in the extracellular matrix, which are essential for proper bone formation and health. The study of PHOSPHO1 recombinant protein has gained attention due to its potential implications in understanding bone-related diseases such as osteoarthritis and osteoporosis. Research suggests that dysregulation of PHOSPHO1 can lead to impaired mineralization and, consequently, abnormalities in bone development. By producing and characterizing recombinant PHOSPHO1, scientists aim to elucidate its biochemical properties, understand its interaction with other proteins, and explore its functional role in cellular processes related to bone metabolism. The recombinant protein serves as a valuable tool for in vitro studies, allowing for the investigation of its enzymatic activity and regulatory mechanisms. Furthermore, insights from PHOSPHO1 research could contribute to the development of targeted therapies for bone disorders, making it a promising focus for ongoing investigations in bone biology and mineralization.
