Cat: PA2000-9272

Recombinant Human METTL2B Protein,His

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Analytical Data

  • Gene name

    METTL2B

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    METTL2BtRNA N(3)-methylcytidine methyltransferase METTL2B; EC 2.1.1.-; Methyltransferase-like protein 2B

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q6P1Q9

  • Expression Region

    2-378 aa

  • AA Sequence

    AGSYPEGAP AILADKRQQF GSRFLSDPAR VFHHNAWDNV EWSEEQAAAA ERKVQENSIQ RVCQEKQVDY EINAHKYWND FYKIHENGFF KDRHWLFTEF PELAPSQNQN HLKDWFLENK SEVCECRNNE DGPGLIMEEQ HKCSSKSLEH KTQTPPVEEN VTQKISDLEI CADEFPGSSA TYRILEVGCG VGNTVFPILQ TNNDPGLFVY CCDFSSTAIE LVQTNSEYDP SRCFAFVHDL CDEEKSYPVP KGSLDIIILI FVLSAVVPDK MQKAINRLSR LLKPGGMVLL RDYGRYDMAQ LRFKKGQCLS GNFYVRGDGT RVYFFTQEEL DTLFTTAGLE KVQNLVDRRL QVNRGKQLTM YRVWIQCKYC KPLLSSTS

  • Molecular Weight

    43,4 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

METTL2B, a member of the methyltransferase family, has garnered attention in recent years due to its potential role in RNA methylation and gene regulation. Research has indicated that METTL2B may influence various biological processes, including cell differentiation, proliferation, and response to stress. Given its involvement in methylation, it is hypothesized that METTL2B can affect mRNA stability and translation, contributing to the fine-tuning of gene expression. Dysregulation of this protein has been implicated in several diseases, including cancer, where altered methylation patterns can drive oncogenic pathways. Consequently, generating recombinant METTL2B protein is pivotal for elucidating its biochemical functions and understanding its role in cellular processes. Through structural and functional analyses of the recombinant protein, researchers aim to explore the mechanisms by which METTL2B interacts with RNA substrates and other cellular factors. This could provide insights into potential therapeutic targets for diseases linked to methylation dysregulation. As a result, the study of METTL2B and its recombinant protein forms an essential avenue for advancing our understanding of epitranscriptomics and its impact on health and disease.

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