Cat: PA1000-2377

Recombinant Human PHB Protein,His

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Analytical Data

  • Gene name

    PHB

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    PHB1;PHB;Prohibitin 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P35232

  • Expression Region

    1-272aa

  • AA Sequence

    MGSSHHHHHH SSGLVPRGSH MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ

  • Molecular Weight

    32 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

PHB (Prohibitin) is a highly conserved protein that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. Recent research has highlighted its involvement in mitochondrial function and cellular signaling pathways, making it an attractive target for therapeutic interventions in diseases such as cancer and neurodegenerative disorders. PHB exists in multiple forms, including cytosolic and mitochondrial localizations, and has been shown to interact with various molecular partners, influencing cell fate decisions. The study of recombinant PHB proteins offers insights into their structure-function relationships and regulatory mechanisms. With advances in recombinant DNA technology, scientists can produce PHB in a controlled environment, facilitating detailed investigations into its biological roles and potential applications in drug development. Understanding the dynamics of PHB through recombinant approaches may pave the way for novel strategies in treating diseases associated with its dysregulation, making it a subject of significant interest in both basic and applied biomedical research.

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