Analytical Data
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Gene name
MED29
- Application
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Alternative Names
MED29; IXL; Mediator of RNA polymerase II transcription subunit 29; Intersex-like protein; Mediator complex subunit 29
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NX70
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Expression Region
1-221aa
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AA Sequence
MLKSNGERRSRNALPAVYARKMAASQQQASAASSAAGVSGPSSAGGPGPQQQPQPPAQLVGPAQSGLLQQQQQDFDPVQRYKMLIPQLKESLQTLMKVAAQNLIQNTNIDNGQKSSDGPIQRFDKCLEEFYALCDQLELCLRLAHECLSQSCDSAKHSPTLVPTATKPDAVQPDSLPYPQYLAVIKAQISCAKDIHTALLDCANKVTGKTPAPPAGPGGTL
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Molecular Weight
49.9 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MED29, a member of the Mediator complex, plays a crucial role in the regulation of gene expression by acting as a co-activator that links transcription factors to the RNA polymerase II machinery. Research into MED29 has gained traction due to its implications in various biological processes, including cell differentiation, development, and responses to environmental stimuli. Abnormalities in MED29 expression or function have been associated with several diseases, including cancer, making it a potential target for therapeutic intervention. Studies have demonstrated that MED29 is involved in modulating the activity of specific transcription factors, influencing the transcriptional landscape of target genes. The exploration of MED29’s structure and its interactions within the Mediator complex has provided insights into its functional mechanisms. Recent advances in techniques such as cryo-electron microscopy and crystallography have facilitated a deeper understanding of the structural basis for MED29’s role in transcription regulation. Given its multifaceted functions and significance in health and disease, MED29 continues to be a subject of intense research, with potential applications in biotechnology and medicine. Understanding the nuances of MED29's mechanisms could pave the way for new therapeutic strategies aimed at correcting dysregulated gene expression associated with various pathologies.
